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Springer Protocols eBook Series

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  • Cell Cycle Control

    Methods and Protocols

    Edited by Anna Castro, Benjamin Lacroix ...
    Series series Springer Protocols
    This detailed volume collects techniques to study the highly regulated cell cycle process. Beginning with chapters investigating these processes and assessing how cells respond when these complicated pathways are simplified by using synthetic biology and in vitro reconstitutions, the book continues by exploring how cells sense and respond to environmental conditions, different model systems and ... Read more

    $143.09 USD

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  • RNA Methodologies

    Laboratory Guide for Isolation and Characterization

    This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of ... Read more

    $116.09 USD

  • PCR Guru

    An Ultimate Benchtop Reference for Molecular Biologists

    PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic ... Read more

    $71.99 USD

  • Drosophila

    Methods and Protocols

    Edited by Christian Dahmann ...
    Series series Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Read more

    $116.99 USD

  • Zebrafish

    Methods and Protocols

    Series series Springer Protocols
    This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the ... Read more

    $179.09 USD

  • DNA-Protein Interactions

    Methods and Protocols

    Edited by Marcos Simoes-Costa ...
    Series series Springer Protocols
    This volume details protocols emphasizing systems-level approaches that can be applied to genomic analyses. Chapters detail techniques for optimized application in in vivo systems, spatial, physiological, environmental contexts, imaging-based techniques, single-molecule approaches, CRISPR systems, new genomic approaches, and measurements of kinetics governing. Written in the format of the highly ... Read more

    $116.99 USD

  • Histones

    Methods and Protocols

    Series series Springer Protocols
    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Read more

    $224.09 USD

  • Dictyostelium discoideum

    Methods and Protocols

    Edited by Alan R. Kimmel ...
    Series series Springer Protocols
    This volume discusses the latest advancements and techniques used to study Dictyostelium discoideum. The chapters in this book cover topics such as cytokinesis, membrane function and damage, and nutrient/bacterial processing; autophagy activation and function; protein localization, interaction, and activation; cell migration and contact guidance for quantification of cytoskeletal dynamics; and ... Read more

    $152.09 USD

  • Mitotic Exit

    Methods and Protocols

    Edited by Victor M. Bolanos-Garcia ...
    Series series Springer Protocols
    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Read more

    $224.09 USD

  • Spatial Genome Organization

    Methods and Protocols

    Edited by Tom Sexton ...
    Series series Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Read more

    $143.09 USD

  • Mitochondrial DNA

    Methods and Protocols

    Series series Springer Protocols
    This volume compiles a comprehensive range of methods to study key aspects of mitochondrial DNA including nucleoid structure and packaging, replication, genome integrity, and disease. Chapters are organized into eight methodological sections that cover in vitro and in vivo methods, including for mtDNA isolation, visualization, deep sequencing, gene editing, and diagnostic aspects of mtDNA disease. ... Read more

    $215.99 USD

  • piRNA

    Methods and Protocols

    Series series Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Read more

    $143.99 USD