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Springer Protocols eBook Series

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  • Histones

    Methods and Protocols

    Series series Springer Protocols
    This second edition provides new and updated chapters on histones for molecular biologists, biochemists and geneticists. Chapters cover protein complexes that modify chromatin either by adding or removing post-translational modifications, or by exchanging histone variants within the nucleosome. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief ... Read more

    $224.09 USD

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  • PCR Guru

    An Ultimate Benchtop Reference for Molecular Biologists

    PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic ... Read more

    $71.99 USD

  • Spatial Genome Organization

    Methods and Protocols

    Edited by Tom Sexton ...
    Series series Springer Protocols
    This detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the ... Read more

    $143.09 USD

  • Optogenetics

    Methods and Protocols

    Edited by Armin Baumschlager ...
    Series series Springer Protocols
    This volume explores the latest advancements in the field of optogenetics and how it uses cellular light-sensing components and genetic engineering to control proteins and biological processes. The book chapters are organized into four parts. Part One focuses on intracellular optogenetic components for control of specific cell functions; Part Two looks at externally supplied light regulators that ... Read more

    $197.09 USD

  • Prokaryotic Gene Regulation

    Methods and Protocols

    Series series Springer Protocols
    This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal ... Read more

    $143.09 USD

  • The Mitoribosome

    Methods and Protocols

    Series series Springer Protocols
    This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Read more

    $215.09 USD

  • mRNA Decay

    Methods and Protocols

    Edited by Maxime Wery ...
    Series series Springer Protocols
    This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as ... Read more

    $251.09 USD

  • Deadenylation

    Methods and Protocols

    Series series Springer Protocols
    This volume provides new approaches and technologies into roles of poly(A) metabolism in translation, RNA stability, and quality control of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and ... Read more

    $161.99 USD

  • piRNA

    Methods and Protocols

    Series series Springer Protocols
    This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high ... Read more

    $143.99 USD

  • Chemical and Genomic Methods in Nucleic Acid Biology

    Series Book 705 - Methods in Enzymology
    Chemical and Genomic Methods in Nucleic Acid Biology, Volume 704 highlights new advances in the field of nucleic acids, with this new volume presenting interesting chapters written by an international board of authors. Specific chapters in this new release include A real-time FRET-based biochemical assay for DNA deaminase enzymology and inhibition, DEER spectroscopy to probe DNA wrapping by ... Read more

    $179.09 USD

  • Mitotic Exit

    Methods and Protocols

    Edited by Victor M. Bolanos-Garcia ...
    Series series Springer Protocols
    This volume explores the latest approaches and techniques used to study mitotic exit in diverse model organisms. The chapters in this book cover topics such as transgenic methods generation of RNAi-sensitive cell lines; gene overexpression in heterologue gene expression systems; quantitative live cell imaging and FRET-FLIM; biochemical protocols for analyzing post-translational modifications ... Read more

    $224.09 USD

  • SMC Proteins

    Methods and Protocols

    Series series Springer Protocols
    The aim of this volume is to provide a wide range of experimental methods for analyzing molecular functions of SMC complexes, such as microscopic imaging, protein localization, chromosome conformation analyses by deep DNA sequencing and biochemical reconstitution with purified proteins or cell lysate. Written in the highly successful Methods in Molecular Biology series format, the chapters include ... Read more

    $179.09 USD