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Springer Protocols eBook Series

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  • Golgi

    Methods and Protocols

    Edited by Yanzhuang Wang, Vladimir V Lupashin, Todd R. Graham ...
    Series series Springer Protocols
    This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study ... Read more

    $215.99 USD

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  • Drosophila

    Methods and Protocols

    Edited by Christian Dahmann ...
    Series series Springer Protocols
    This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and ... Read more

    $116.99 USD

  • The Design and Development of Novel Drugs and Vaccines

    Principles and Protocols

    Edited by Tarun Kumar Bhatt, Surendra Nimesh ...
    The Design and Development of Novel Drugs and Vaccines: Principles and Protocols presents both in silico methods and experimental protocols for vaccine and drug design and development, critically reviewing the most current research and emphasizing approaches and technologies that accelerate and lower the cost of product development. Sections review the technologies and approaches used to identify, ... Read more

    $134.99 USD

  • Antibody Engineering Volume 1

    Edited by Roland E. Kontermann, Stefan Dübel ...
    Series series Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Read more

    $332.09 USD

  • Mitochondrial Medicine

    Volume 2: Assessing Mitochondria

    Edited by Volkmar Weissig, Marvin Edeas ...
    Series series Springer Protocols
    This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D ... Read more

    $107.99 USD

  • Membrane Trafficking

    Methods and Protocols

    Edited by Jingshi Shen ...
    Series series Springer Protocols
    This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma ... Read more

    $143.99 USD

  • Peroxisomes

    Methods and Protocols

    Edited by Michael Schrader ...
    Series series Springer Protocols
    This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast ... Read more

    $197.99 USD

  • Single Cell ‘Omics of Neuronal Cells

    Edited by Jonathan V. Sweedler, James Eberwine, Scott E. Fraser ...
    Series series Springer Protocols
    This volume discusses the latest techniques used in the diverse fields of single cell ‘omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical ... Read more

    $143.09 USD

  • The ESCRT Complexes

    Methods and Protocols

    Edited by Emmanuel Culetto, Renaud Legouis ...
    Series series Springer Protocols
    This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering ... Read more

    $98.99 USD

  • HDAC/HAT Function Assessment and Inhibitor Development

    Methods and Protocols

    Edited by Oliver H. Krämer ...
    Series series Springer Protocols
    This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors ... Read more

    $170.09 USD

  • The Immune Synapse

    Methods and Protocols

    Edited by Cosima T. Baldari, Michael L. Dustin ...
    Series series Springer Protocols
    This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as ... Read more

    Was $242.99 USD Now $179.99 USD

  • Proximity Labeling

    Methods and Protocols

    Edited by Murat Sunbul, Andres Jäschke ...
    Series series Springer Protocols
    This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity ... Read more

    $125.09 USD