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Springer Protocols eBook Series

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  • The Ubiquitin Code

    Edited by Manuel S Rodriguez, Rosa Barrio ...
    Series series Springer Protocols
    This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity ... Read more

    $116.99 USD

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  • Cell Biology Assays

    Proteins

    Edited by Fanny Jaulin, Cedric Espenel ...
    Protein assay methods are used for protein identification with blood groups, cell surface markers, drugs and toxins. This text features comprehensive protocols essential for researchers studying various areas of biological and medical sciences. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at ... Read more

    $96.29 USD

  • Phage Display of Peptides and Proteins

    A Laboratory Manual

    Edited by Brian K. Kay, Jill Winter, John McCafferty ...
    Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, ... Read more

    $54.89 USD

  • Alternative pre-mRNA Splicing

    Theory and Protocols

    Edited by Stefan Stamm, Chris Smith, Reinhard Lührmann ...
    This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing intwelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative ... Read more

    $108.00 USD

  • Antibody Engineering Volume 1

    Edited by Roland E. Kontermann, Stefan Dübel ...
    Series series Springer Protocols
    Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. "Antibody Engineering" provides a comprehensive toolbox covering the well-established basics but also many exciting new ... Read more

    $332.09 USD

  • Peptides

    Chemistry and Biology

    by Norbert Sewald, Hans-Dieter Jakubke ...
    Thoroughly updated, incorporating around 25 % new material, Sewald/Jakubke remains the only modern and scientifically up-to-date advanced textbook on peptide biochemistry, distilling the knowledge of hundreds of publications into a highly readable synopsis of this diverse field.The authors explain the broad fundamentals of peptide synthesis and structure, systematically addressing important ... Read more

    $142.00 USD

  • Molecular Analysis and Genome Discovery

    Edited by Ralph Rapley, Stuart Harbron ...
    Molecular Analysis and Genome Discovery, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and ... Read more

    $124.00 USD

  • Plant Proteostasis

    Methods and Protocols

    Edited by L. Maria Lois, Marco Trujillo ...
    Series series Springer Protocols
    This second edition compiles state-of-art protocols to study the molecular mechanisms that determine proteome composition and contribute to maintaining protein homeostasis. Chapters describe methods that range from the in vitro analysis of enzyme cascades to real-time in vivo studies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their ... Read more

    $143.99 USD

  • The Mitoribosome

    Methods and Protocols

    Edited by Antoni Barrientos, Flavia Fontanesi ...
    Series series Springer Protocols
    This volume provides classic and new methods to study the structure, assembly pathway, and protein synthesis ability of mitoribosomes across species. Following an introduction of fundamental concepts on the topic, method chapters present detailed protocols based on cryo-electron tomography, cryo-EM approaches, mitoribosome purification techniques, mitochondrial translation assays, and methods to ... Read more

    $215.09 USD

  • Chaperones

    Methods and Protocols

    Edited by Stuart K. Calderwood, Thomas L. Prince ...
    Series series Springer Protocols
    This second edition volume expands on the previous edition with new discussions on the latest techniques used to study molecular chaperones and the stress response. The chapters in this book cover such as analysis of the initiation and regulation of the stress response; the role of heat shock protein 90 (Hsp90) in gene expression through chromosome-immunoprecipitation; features of chaperone ... Read more

    $161.99 USD

  • Enzyme-Mediated Ligation Methods

    Edited by Timo Nuijens, Marcel Schmidt ...
    Series series Springer Protocols
    This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and ... Read more

    $107.99 USD

  • RNA-Protein Complexes and Interactions

    Methods and Protocols

    Edited by Ren-Jang Lin ...
    Series series Springer Protocols
    This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in ... Read more

    $215.99 USD